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Skin Corrosion (OECD TG 431)
Skin corrosion refers to the production of irreversible tissue damage in the skin following the application of a test material. The potential for chemical-induced skin corrosion is an important consideration in establishing procedures for the safe handling, packing, and transport of chemicals.
The EpiDerm Skin Corrosion Test is able to reliably discriminate chemicals that are corrosive to skin from non-corrosive chemicals and can be used for the classification of skin corrosion hazard according to the GHS System adopted by the OECD.
MatTek offers the EpiDerm Skin Corrosion Test as a GLP or non-GLP service.
Protocol
| Test Model | EpiDerm |
| Replicates | N=2 tissues per test condition |
| Test Article Quantity | 1ml or 1g |
| Assay Controls | Negative Control – Ultrapure H2O Positive Control – 8N Potassium Hydroxide |
| Endpoints | MTT Tissue Viability Assay |
| Data Delivery | % relative viability ± SD In vitro classification |
Download the Skin Corrosion Test Protocol
Data
| Mean Tissue Viability (% of NC) | Classification |
|---|---|
| 3 minute < 50% | Corrosive |
| 3 minute ≥ 50% and 1 hour < 15% | Corrosive |
| 3 minute ≥ 50% and 1 hour ≥ 15% | Non-Corrosive |
References
OECD (2001) Harmonised Integrated Classification System for Human Health and Environmental Hazards of Chemical Substances and Mixtures. OECD Series on Testing and Assessment Number 33. ENV/JM/MONO(2001)6, Paris http://www.olis.oecd.org/olis/2001doc.nsf/LinkTo/env-jm-mono(2001)6
Fentem, J.H., Archer, G.E.B., Balls, M., Botham, P.A., Curren, R.D., Earl, L.K., Esdaile, D.J., Holzhutter, H.-G., and Liebsch, M. (1998). The ECVAM international validation study on in vitro tests for skin corrosivity. 2. Results and evaluation by the Management Team. Toxicol. in Vitro 12, 483-524
Liebsch, M., Traue, D., Barrabas, C., Spielmann, H., Uphill, P., Wilkins, S., McPherson, J.P., Wiemann, C., Kaufmann, T., Remmele, M. and HolzhĂĽtter, H.-G (2000). The ECVAM prevalidation study on the use of EpiDerm for skin corrosivity testing. ATLA 28, pp. 371-401.
OECD (2004). Guidelines for the Testing of Chemicals, No. 431: In Vitro Skin Corrosion: Human Skin Model Test. Paris, France: OECD, 8 p.
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