Visit Us at the 10th Annual World Congress on Alternatives and Animal Use in the Life Sciences    Â
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Session II: Lessons Learned
CON4EI: EpiOcular Eye Irritation Tests – OECD TG 492 and ET-50 (time-to-toxicity) Protocols (Abstract 558 Poster M-A39)
Monday 8/21 12:15 pm – 1:15 pm
Silvia Letasiova. MatTek IVLSL, Bratislava, Slovakia.
Session III: Innovative Models for Safety and Efficacy – Respiratory Models
Development of an In Vitro Alternative Method for Acute Inhalation Toxicity Testing (Abstract 497 Poster T-A64)
Tuesday 8/22 12:00 pm – 1:15 pm
Patrick Hayden. MatTek Corporation, Ashland, MA.
Detection of Reactive Chemicals and Oxidants Using an Organotypic Human Airway Model with Nrf2 Reporter Activity (EpiAirway – Nrf2): Application to Evaluation of Tobacco Products (Abstract 666 Poster T-A67)
Session III: Innovative Models for Safety and Efficacy – Respiratory Models
Tuesday 8/22 12:00 pm – 1:15 pm
Patrick Hayden. MatTek Corporation, Ashland, MA.
Session III: Innovative Models for Safety and Efficacy – Liver
Use of Mass Spectrometry Imaging and a Full Thickness 3D Skin Equivalent (EpiDermFT) for Evaluation of Percutaneous Absorption (Abstract 658 Poster T-B59)
Tuesday 8/22 12:00 pm – 1:15 pm
Michael Bachelor. MatTek Corporation, Ashland, MA.
Session III: Innovative Models for Safety and Efficacy – Ocular Models
Molecular Mechanisms of Corneal Oxidative Stress: In Vitro Reconstructed Human Corneal Tissue Model (EpiCorneal) (Abstract 654 Poster T-A54)
Tuesday 8/22 12:00 pm – 1:15 pm
Yulia Kaluzhny. MatTek Corporation, Ashland, MA.
Session III: Innovative Models for Safety and Efficacy – Skin
Determination of Contact Sensitization Potential of Chemicals Using In Vitro Reconstructed Normal Human Epidermal Model EpiDerm: Impact of the Modality of Application (Abstract 483 Poster T-B22)
Tuesday 8/22 12:00 pm – 1:15 pm
Silvia Letasiova. MatTek IVLSL, Bratislava, Slovakia.
Assessment of the Phototoxicity of Three Different TiO2 Nano-Forms Using Reconstructed Human Tissue Model EpiDerm (Abstract 552 Poster T-B24)
Tuesday 8/22 12:00 pm – 1:15 pm
Silvia Letasiova. MatTek IVLSL, Bratislava, Slovakia.
Hyperosmolal Vaginal Lubricants Markedly Increase Epithelial Damage in a 3D Vaginal Epithelium Model (EpiVaginal) (Poster T-B26)
Tuesday 8/22 12:00 pm – 1:15 pm
Seyoum Ayehunie. MatTek Corporation, Ashland, MA.
Attend a talk by Dr. Seyoum Ayehunie
Use of 3D-Human Small Intestinal Tissue Model (EpiIntestinalTM) as an Alternate to Animal Testing to Predict Drug-Induced Gastrointestinal (GI) Toxicity.
Session III-17: New Advances in Intestinal Models
Monday 8/21 Session time: 1:15 pm -2:45 pm Presentation Time: 2:00 pm – 2:15 pm
Washington State Convention Center, Rooms 619-620
Abstract 601 Program Number III-17-601
Timothy Landry1, Seyoum Ayehunie1, Zachary Stevens1, Matthew Wagoner2, Mitchell Klausner1, and Patrick Hayden1. 1MatTek Corporation, Ashland, MA, 2AstraZeneca, Waltham, MA.
The utility of an in vitro primary human cell based small intestinal 3D tissue (SMI) model as an investigational tool for drug induced GI toxicity was evaluated. A blinded study was performed using N=8 therapeutic compounds (5 problematic and 3 well-tolerated drugs in humans) for which dog and rat toxicity studies were not predictive of human outcome. MTT viability, tissue barrier integrity using transepithelial electrical resistance (TEER) measurements and histology were used as end points. Results showed that the SMI tissue detected drug-induced reduction in TEER in 5/5 of the problematic drugs at concentrations within or below 30x clinical Cmax.  Importantly, the SMI system showed no effect within 1,000x clinical exposure levels for the three well-tolerated drugs. Overall, the use of TEER and histology as endpoints make the SMI tissue a sensitive predictive tool to assess drug-induced GI toxicity. The use of the SMI tissues for GI toxicity testing is cost effective and reduces animal use.
Schedule a meeting with Dr. Ayehunie at the conference
