PermaCell Culture Inserts

MatTek PermaCell Culture Inserts are sterile, tissue culture devices that are ideal for the growth/differentiation of cells and 3D organotypic culture. The 8mm inserts are uniquely designed to function as either standing or hanging inserts, allowing researchers increased flexibility when designing studies. The inserts are sold in 24-well plates (24 inserts/plate), but can be used in 6-, 12-, and 24-well culture plate formats. Various membrane types allow researchers to select inserts for specific needs, such as cell attachment, differentiation, organotypic 3D culture, drug transport/permeability, and imaging studies.

PermaCell inserts are specially designed for 3D organotypic tissue culture and are easily adaptable to TEER measurements and other analytical techniques.

Download the PermaCell Spec Sheet

8mm DOWNLOAD

Download the PermaCell Application Note

8mm DOWNLOAD

8mm Clear PTFE Inserts DOWNLOAD

Description

8mm Inserts

  • 24 8mm inserts packaged in 24-well plate
  • Hang-top lid for airlifting can be purchased separately for use in 12- or 24-well formats
  • Pore size of 0.4µm or 8µm, custom pore sizes available
  • PTFE, PET, PC membrane, and custom membranes available
    • PTFE membranes have a high pore density and are clear in culture, which allows for visualization of cells via brightfield and fluorescent microscopy
  • TEER compatible

A 3D render of a plastic cylinder with a membrane bottom and open top.

 

Compatible TEER Electrode: STX HTS EVOM™ Electrode for TEER in PermaCell Culture Insert Plate

Need an EVOM² Adapter? STX4 Electrode Adapter Cable to Use with EVOM2

Specifications

8mm Insert Specifications
Height (including feet)8.8 mm
Height of Feet0.8 mm
Outer Diameter13.6 mm
Inner Diameter8.8 mm
Membrane Area0.6 cm2
Membrane Pore Sizes0.4, 8 µm
Solvent CompatibilitySame as polystyrene
SterilityGamma Irradiation, shipped sterile in 24-well plates
Membrane TypesPolyethelyene terephthalate (PET), Polytetrafluorethylene (PTFE), Polycarbonate (PC)

Membrane Types

    • Polycarbonate (PC)
    • Polyethylene terephthalate (PET)
    • Teflon (PTFE)
      • clear in culture
    • Specialty membranes also available
SEM membrane images. A. Polycarbonate (PC) membrane, B. Polyethylene terephthalate (PET) membrane, C. Teflon (PTFE) membrane

Coatings and Special Orders

MatTek offers PET, PC, PTFE as standard membrane options. Many cell types will grow directly in PermaCell Insert and Insert Plates. For cell types that require an extracellular matrix coating, we recommend the following special order coatings from Advanced BioMatrix:

PureCol® Type I Collagen https://advancedbiomatrix.com/purecol/

Type IV Collagen https://advancedbiomatrix.com/typeiv-lyophilized.html

Fibronectin https://advancedbiomatrix.com/fibronectin-solution.html

Vitronectin https://advancedbiomatrix.com/vitronectin-solution.html

Poly-D-Lysine https://advancedbiomatrix.com/polydlysinesolution.html

Poly-L-Lysine https://advancedbiomatrix.com/polyllysinesolution.html

Poly-L-Ornithine https://advancedbiomatrix.com/polylornithinesolution.html

 

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