EVALUATION OF THE EFFECTS OF GOLDEN SILK-DERIVED EXTRACT ON EPIDERMAL BARRIER MARKERS, CASPASE-14 AND RELATED GENES IN HUMAN SKIN EQUIVALENT MODELS.

Glucocorticoids are widely used for their anti-inflammatory effects but the mechanism of their action in skin has not been fully understood. Bayo et al reported that glucocorticoid receptor is required for skin barrier competence(1) and Cirillo et al reported that epidermal keratinocyte synthesize and activate cortisol(2). Caspase-14 is a cystenyl-aspartate-specific proteinase that is specifically expressed in epidermal keratinocytes. Dysregulation of caspase-14 expression is implicated in impaired skin barrier formation(3). In the current study, we used reconstructed epidermal model, EPI-201. This 3D tissue consists of normal, human-derived epidermal keratinocytes which have been cultured to form a multilayered, metabolically and mitotically active, differentiating model of the human epidermis. We have characterized the expression of caspase-14 and evaluated the effects of natural compounds on the expression of caspase-14(4).

Caspase-14, elafin, EPI-100-MM-HCF, EPI-201, glucocorticoid receptor, glucocorticoid receptor antagonist, RU-486, Involucrin, profilaggrin, skin barrier, transglutaminase 1

Brown Algae, lipid soluble extract, Brown Algae, water soluble extract, dexamethasone, Galactomyces ferment filtrate, glucocorticoid receptor antagonist, RU-486, Golden Silk-Derived Extract