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FILTER-WELL TECHNOLOGY FOR ADVANCED THREE-DIMENSIONAL CELL CULTURE: PERSPECTIVES FOR RESPIRATORY RESEARCH.

Berube1, K., Pitt2, A., Hayden3, P., Prytherch­1, Z. and Job1, C. 1School of Biosciences, Cardiff University, Cardiff, Wales, UK; 2Millipore Corporation, Danvers, MA, USA; 3MatTek Corporation, Ashland, MA, USA.
Abstract

Cell culture has long been a valuable tool for studying cell behaviour. Classical plastic substrates are two-dimensional, and usually promote cellular proliferation and inhibit differentiation. Understanding cell behaviour within complex multicellular tissues requires the systematic study of cells within the context of specific model microenvironments. A model system must mimic, to a certain degree, the in vivo situation, but, at the same time, can significantly reduce its complexity. There is increasing agreement that moving up to the third dimension provides a more physiologically-relevant and predictive model system. Moreover, many cellular processes (morphogenesis, organogenesis and pathogenesis) have been confirmed to occur exclusively when cells are ordered in a three-dimensional (3-D) manner. In order to achieve the desired in vivo phenotype, researchers can use microporous membranes for improved in vitro cell culture experiments. In the present review, we discuss the applications of filter-well technology for the advanced 3-D cell culture of human pulmonary cells.

Keywords

AIR-100 , Airway drug development , Airway toxicology , Asthma research , Ciliogenesis , CLCA1 (calcium-activated chloride channel regulator 1) , EpiAirway FT , EpiAirwayTM , Goblet cell hyperplasia , Good Manufacturing Practices (GMP) , Influenza , Long term culture , Mucin 5AC , Mucin goblet cells, Periodic acid-Schiff staining , Rhinovirus , TEER , TFF3 (trefoil factor 3) , Th2 (Type 2 T-helper cell) cytokines

Materials Tested

IL-13, IL-4 , Triton X-I00 (0.2%)

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