IN VITRO SKIN BIOMARKER RESPONSES TO A NEW ANTI-AGING PEPTIDE, PAL-KT.

This study by scientists at Procter & Gamble (P&G Beauty Div.) and Sederma demonstrated that MatTek’s EpiDermFT full-thickness in vitro human skin tissue equivalent is an excellent in vitro model for screening novel peptides for their potential anti-aging effects on facial skin. Peptides applied topically in moisturizer-type cosmetic products are known to improve the appearance of signs of facial skin aging. For example, palmitoyl-KTTKS pentapeptide has been shown to improve the appearance of fine lines and wrinkles in aging facial skin when applied twice daily in a facial moisturizer. A screening program was undertaken to identify additional peptides that may improve the appearance of aging facial skin. A peptide identified via the screening program was Pal-KT (palmitoyl-lysine-threonine), which proved to have activity superior to other peptides tested in human derived fibroblasts and human skin equivalents on skin structure-related biomarkers. Pal-KT, a palmitoylated dipeptide, increased in vitro expression of skin biomarkers indicative of effects on epidermal keratinocyte differentiation (keratin 10, desmoglein), dermal fibroblasts (vimentin), dermal matrix (collagens, elastin) including dermal matrix remodeling (MMPs and TIMPs), basement membrane (laminins, collagen IV), hydration (aquaporin), wound healing (fibronectin) and longevity (sirtuin). Thus, Pal-KT produces promising in vitro skin responses that are widely believed to be consistent with skin anti-aging benefits.

Anti-aging, Basement membrane, CD44, Collagen I, Collagen III, Collagen IV, Collagen-VI, Dermal matrix components, Dermal remodeling, EFT-400, Elastin, EpiDerm-FT, EpiDermFT, Facial skin aging, Fibronectin, Fibronectin, Hyaluronic acid, Laminin 1, Laminin IV, MMP, MMP-1, MMP-2, Palmitoylated dipeptide, Procollagen, Procollagen C, TIMP1, TIMP2, Vimentin

Palmitoyl-lysine-threonine