Reference Libraries

February 14, 2025

Two- and Three-Dimensional Culture Systems: Respiratory In Vitro Tissue Models for Chemical Screening and Risk-Based Decision Making

Risk of lung damage from inhaled chemicals or substances has long been assessed using animal models. However, New Approach Methodologies (NAMs) that replace, reduce, and/or refine the use of animals in safety testing such as 2D and 3D cultures are... Read More

January 29, 2025

Assessing nanotoxicity of food-relevant particles: A comparative analysis of cellular responses in cell monolayers versus 3D gut epithelial cultures

Engineered nanoparticles (NPs) are extensively used in the food industry, yet safety concerns remain. The lack of validated methodologies is a bottleneck towards resolving this uncertainty. Hence, the current study aims to compare two cell models by examining the toxicological... Read More

January 28, 2025

Cooperation of Cysteine, Ascorbic Acid, Pyridoxine, and α-Lipoic Acid in Antioxidant Effects on Human Keratinocytes and 3D Human Skin Models

Combinations of cysteine, ascorbic acid, and pyridoxine are frequently used in oral formulations. Although there have been many reports on the efficacy of each of these ingredients, little information is known about their combined effects on skin cells. The purpose... Read More

January 28, 2025

Automated Transepithelial Electrical Resistance (TEER) Measurements Allow for Rapid Screening of the Gastrointestinal Toxicity Profile of Therapeutics

Gastrointestinal toxicity (GIT) ranks among the most common clinical side effects for many orally administered pharmaceuticals, in particular for oncology drugs which are administered over an extended time period. Previously, we utilized an in vitro small intestine (SMI) microphysiological tissue... Read More

January 23, 2025

Human keratinocyte caspase-14 expression is altered in human epidermal 3D models by dexamethasone and by natural products used in cosmetics

Caspase-14 is a cysteinyl-aspartate-specific proteinase that is specifically expressed in epidermal keratinocytes. Dysregulation of caspase-14 expression is implicated in impaired skin barrier formation. To elucidate the regulation of caspase-14 in differentiated keratinocytes, we characterized the expression of caspase-14 in normal... Read More