Reference Libraries

December 11, 2015

β-GLUCOCEREBROSIDASE ACTIVITY IN MAMMALIAN STRATUM CORNEUM.

Although previous studies have demonstrated a crucial role for the enzyme â-glucocerebrosidase (GlcCer’ase) in the final steps of membrane structural maturation in mammalian stratum corneum (SC) and epidermal homeostasis, the precise in vivo localization of GlcCer’ase activity and protein is... Read More
December 11, 2015

SIMULTANEOUS DETECTION AND SEMIQUANTIFICATION OF DNA DAMAGE IN NORMAL AND APOPTOTIC CELLS: TRIPLE-IMMUNOFLUORESCENT LABELING USING DAPI, ANTIBODIES, AND TUNEL.

We developed a triple-labeling immunofluorescence technique that simultaneously identifies total DNA (DAPI), DNA damage (antibodies), and dead cells [terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL)-positive cells] and a method that semiquantifies DNA damage in paraffin-embedded tissues. Using this technique in... Read More