Toxicity and Inflammatory Response to Common Magic Mouthwashes in a 3D Oral Reconstructed Tissue Model

Magic Mouthwash is a general term describing oral rinses prescribed by a doctor for the treatment of pain, inflammation, or infection, commonly as a result of chemotherapy and radiation induced oral sores (oral mucositis). There is no set combination of ingredients for the preparation of a magic mouthwash, rather they are formulated for the individual needs of each patient, selecting from an array of various active ingredients. Although the ingredients most commonly used to formulate Magic Mouthwashes are considered safe as prescribed, the final formulations lack toxicity and inflammatory response data. Four common Magic Mouthwash formulations (MM 1,2,3,4), which vary slightly in active and inactive ingredients, were tested for cytotoxicity and inflammatory response using a novel testing strategy based on a commercially available (3D) EpiOralTM tissue model (MatTek Corporation, Ashland, MA), reconstructed from normal human-derived oral epithelial cells. This in vitro model can be used to accurately and reproducibly assess the safety of products designed to treat oral mucosal conditions and thus reduce the burden of clinical studies

EpiOral (ORL-200), cytotoxicity, inflammatory response, magic mouthwash, Mylanta (33%), Aluminium hydroxide, Al(OH)3, magnesium hydroxide, Mg(OH)2, Simethicone, Lidocaine, Diphenhydramine, Dexamethasone, Hydrocortisone, Nystatin, Prednisolone, antacid, antifungal, antihistamine, anti-inflammatory, anesthetic, MTT, IL-1b

Mylanta (33%), Aluminum hydroxide, Al(OH)3, magnesium hydroxide, Mg(OH)2, Simethicone, Lidocaine, Diphenhydramine, Dexamethasone, Hydrocortisone, Nystatin, Prednisolone